Purity and Characterize the Killer Proteins Produced by Saccharomyces Cerevisiae Strains

Identified killer toxin proteins from Saccharomyces cerevisiae and characterized by HPLC, Therefore, electrophoresis was performed to extract and characterize the protein on a polyacrylamide gel by method SDS – PAGE,The protein bands appeared on the gel, and determined was k28 which secreted by S. cerevisiae depend on molecular weight, which was estimated at 5 kilodalton. It was used in subsequent studies, and the confirmed this toxin by using HPLC which compared with the standard sample. Using bioinformatics In the analysis of the results, were identified the atomic structure and the crystalline shape of the three-dimensional protein by Pymol analysis program and also determined the K28 protein sequence which consists of 2040 nucleotides that code for the amino acids that forming the protein, and then translated protein sequence into the amino acids that forming the protein. It have ability to kill other yeast species and control yeasts that cause the spoilage of beverages and industrial foods.