Assessment of dermal safety of petroleum ether extract from <i>Acorus calamus</i> L

<i>Acorus calamus L</i> (<i>A. calamus</i>), known for its aromatic leaves and rhizomes, is extensively used in traditional medicine to treat digestive issues, inflammation, pain, anxiety, and stress. The petroleum ether extract of <i>A. calamus</i> isolates specific bioactive compounds using petroleum ether, a nonpolar solvent effective in dissolving nonpolar plant compounds. This extract potentially offers antimicrobial, anti-inflammatory, and analgesic benefits. However, research on this extract is limited, necessitating further investigation to determine its safety, efficacy, and medical applications. This study aims to develop more efficient extraction methods for the petroleum ether extract, focusing on sesquiterpenes and isocalamendiol from <i>A. calamus</i>. Additionally, the study will evaluate the extract's safety for topical use, providing insights into potential side effects and contraindications. This information is crucial for assessing the extract's suitability for topical treatments and guiding the development of new therapies and products.