An improvised one-step OptiPrep cushion ultracentrifugation method for outer membrane vesicles isolation of Klebsiella pneumoniae
Our study demonstrates the advantages of the DDGC method in extracting K. pneumoniae OMVs, showing improvements in morphology, particle size distribution, protein content, and biological activity. This provides a solid foundation for further exploration of the biological functions of OMVs and their potential applications in the biomedical field.
Authors
- Yifan Wu (ORCID: https://orcid.org/0000-0002-2938-8323)
- Tianxin Xiang (ORCID: https://orcid.org/0000-0002-1673-6077)
- Ming Lü
- Yang Liu (ORCID: https://orcid.org/0000-0001-5553-3646)
- Ping Li (ORCID: https://orcid.org/0000-0001-6505-645X)
- Wei Zhang (ORCID: https://orcid.org/0000-0002-8777-0600)
- Lanlan Zhu (ORCID: https://orcid.org/0009-0003-1190-6588)
- Tingxiu Peng
Institutions
- Nanchang University (CN)
- China-Japan Friendship Hospital (CN)
- First Affiliated Hospital of Jiangxi Medical College (CN)
- First Affiliated Hospital of Nanchang University (CN)
Publication Details
- Journal
- BMC Microbiology
- Published
- 2024-12-28
- DOI
- https://doi.org/10.1186/s12866-024-03649-y
- Citations
- 3
- Primary Topic
- Bacterial Infections and Vaccines
- Type
- article
- Field-Weighted Citation Impact
- 1.03
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Jiangxi Province
- Nanchang University
- Health Commission of Jiangxi Province