Genomic analysis of DS-1-like human rotavirus A strains uncovers genetic relatedness of NSP4 gene with animal strains in Manhiça District, Southern Mozambique
Post rotavirus vaccine introduction in Mozambique (September 2015), we documented a decline in rotavirus-associated diarrhoea and genotypes changes in our diarrhoeal surveillance spanning 2008-2021. This study aimed to perform whole-genome sequencing of rotavirus strains from 2009 to 2012 (pre-vaccine) and 2017-2018 (post-vaccine). Rotavirus strains previously detected by conventional PCR as G2P[4], G2P[6], G3P[4], G8P[4], G8P[6], and G9P[6] from children with moderate-to-severe and less-severe diarrhoea and without diarrhoea (healthy community controls) were sequenced using Illumina MiSeq<sup>®</sup> platform and analysed using bioinformatics tools. All these G and P-type combinations exhibited DS-1-like constellation in the rest of the genome segments as, I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis revealed that strains from children with and without diarrhoea clustered together with other Mozambican and global strains. Notably, the NSP4 gene of strains G3P[4] and G8P[4] in children with diarrhoea clustered with animal strains, such as bovine and caprine, with similarity identities ranging from 89.1 to 97.0% nucleotide and 89.5-97.0% amino acids. Our findings revealed genetic similarities among rotavirus strains from children with and without diarrhoea, as well as with animal strains, reinforcing the need of implementing studies with One Health approach in our setting, to elucidate the genetic diversity of this important pathogen.
Authors
- Marcelino Garrine (ORCID: https://orcid.org/0000-0002-8619-8761)
- Tacilta Nhampossa (ORCID: https://orcid.org/0000-0002-4616-8752)
- Eva D. João (ORCID: https://orcid.org/0000-0001-7447-9278)
- Peter N. Mwangi (ORCID: https://orcid.org/0000-0002-4732-2811)
- Celso Cunha (ORCID: https://orcid.org/0000-0003-4745-3819)
- Pedro L. Alonso (ORCID: https://orcid.org/0000-0003-3292-3443)
- Augusto Messa (ORCID: https://orcid.org/0000-0002-1794-2462)
- Karen L. Kotloff (ORCID: https://orcid.org/0000-0003-1808-6431)
- Milton T. Mogotsi (ORCID: https://orcid.org/0000-0002-6755-1945)
- Inácio Mandomando (ORCID: https://orcid.org/0000-0002-1078-2187)
- Percina Chirinda (ORCID: https://orcid.org/0000-0002-2649-1034)
- Martin M. Nyaga (ORCID: https://orcid.org/0000-0002-5017-5584)
- Goitom Weldegebriel (ORCID: https://orcid.org/0000-0002-0535-3484)
- Umesh D. Parashar (ORCID: https://orcid.org/0000-0002-9684-9425)
- Jacqueline E. Tate (ORCID: https://orcid.org/0000-0002-8114-6196)
- Filomena Manjate (ORCID: https://orcid.org/0000-0003-3632-2047)
- Sozinho Acácio (ORCID: https://orcid.org/0000-0002-3173-6446)
- Nélio Nobela
- Delfino Vubil (ORCID: https://orcid.org/0000-0001-9139-5594)
- James P. Nataro (ORCID: https://orcid.org/0000-0003-4159-0097)
- Jason M. Mwenda
Institutions
- University of Maryland, Baltimore (US)
- Centers for Disease Control and Prevention (US)
- University of the Free State (ZA)
- Departament de Salut (ES)
- Instituto de Medicina Tropical (PY)
- Manhiça Health Research Centre (MZ)
- Instituto Nacional de Saúde (MZ)
- World Health Organization Regional Office for Africa (CG)
- Barcelona Institute for Global Health (ES)
- University of Virginia (US)
- Universitat de Barcelona (ES)
Publication Details
- Journal
- Scientific Reports
- Published
- 2024-12-28
- DOI
- https://doi.org/10.1038/s41598-024-79767-4
- Citations
- 3
- Primary Topic
- Viral gastroenteritis research and epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 1.17
Funders
- United States Agency for International Development
- Bill and Melinda Gates Foundation
- GAVI Alliance
- World Health Organization
- Centers for Disease Control and Prevention Foundation
- Universiteit van die Vrystaat
- Universidade Nova de Lisboa
- Centers for Disease Control and Prevention
- Fundação para a Ciência e a Tecnologia
- Agencia Española de Cooperación Internacional para el Desarrollo
- Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa