Characterization of Mesenchymal and Neural Stem Cells Response to Bipolar Microsecond Electric Pulses Stimulation
In the tissue regeneration field, stem cell transplantation represents a promising therapeutic strategy. To favor their implantation, proliferation and differentiation need to be controlled. Several studies have demonstrated that stem cell fate can be controlled by applying continuous electric field stimulation. This study aims to characterize the effect of a specific microsecond electric pulse stimulation (bipolar pulses of 100 µs + 100 µs, delivered for 30 min at an intensity of 250 V/cm) to induce an increase in cell proliferation on mesenchymal stem cells (MSCs) and induced neural stem cells (iNSCs). The effect was evaluated in terms of (i) cell counting, (ii) cell cycle, (iii) gene expression, and (iv) apoptosis. The results show that 24 h after the stimulation, cell proliferation, cell cycle, and apoptosis are not affected, but variation in the expression of specific genes involved in these processes is observed. These results led us to investigate cell proliferation until 72 h from the stimulation, observing an increase in the iNSCs number at this time point. The main outcome of this study is that the microsecond electric pulses can modulate stem cell proliferation.
Authors
- Francesca Apollonio (ORCID: https://orcid.org/0000-0001-6937-9731)
- Claudia Consales (ORCID: https://orcid.org/0000-0002-7805-4493)
- Micol Colella (ORCID: https://orcid.org/0000-0003-1715-9619)
- María Pedraza (ORCID: https://orcid.org/0000-0002-1973-3269)
- M. Balucani (ORCID: https://orcid.org/0000-0001-7045-7063)
- Marina Sánchez-Petidier (ORCID: https://orcid.org/0000-0002-5566-2664)
- Francesca Camera (ORCID: https://orcid.org/0000-0001-6455-4094)
- Laura Caramazza (ORCID: https://orcid.org/0000-0002-9304-3477)
- Franck M. André (ORCID: https://orcid.org/0000-0001-6297-1433)
- Micaela Liberti (ORCID: https://orcid.org/0000-0002-7494-2696)
- Paolo Marracino (ORCID: https://orcid.org/0000-0003-4742-8170)
- Vincenzo Palma (ORCID: https://orcid.org/0000-0002-9942-7017)
- Giorgia Innamorati
- Victoria Moreno‐Manzano (ORCID: https://orcid.org/0000-0002-6035-9491)
- Caterina Merla (ORCID: https://orcid.org/0000-0001-8612-9566)
- Giulia Bergafora
- Camilla Codazzi
Institutions
- National Agency for New Technologies, Energy and Sustainable Economic Development (IT)
- University of Rome Tor Vergata (IT)
- Centre National de la Recherche Scientifique (FR)
- Université Paris-Saclay (FR)
- Institut Gustave Roussy (FR)
- Hospital Nacional de Parapléjicos (ES)
- Centro de Investigacion Principe Felipe (ES)
- Sapienza University of Rome (IT)
Publication Details
- Journal
- International Journal of Molecular Sciences
- Published
- 2024-12-27
- DOI
- https://doi.org/10.3390/ijms26010147
- Citations
- 5
- Primary Topic
- Microbial Inactivation Methods
- Type
- article
- Field-Weighted Citation Impact
- 1.93