An innovative ear transplantation for vascularized composite allotransplantation research in porcine model

Vascularized composite allotransplantation (VCA) represents a clinical challenge for transplant therapy, as it involves different tissues with unique immunogenicity. Even when receiving immunosuppressive therapy, they are more vulnerable to severe hypoxia, microvascular damage, and ultimately the rejection or chronic graft dysfunction after transplantation. This study aimed to develop a surgical protocol for VCA of the ear in a porcine biomodel in the absence of immunosuppression, maintaining the in vitro co-culture of the allograft and assessing their relationship with allograft survival. We employed four crossbred pigs and four outbred mini pig biomodels (Sus scrofa), as donors and recipients, to perform four VCAs. Blood samples were taken from each biomodel for crossmatch testing and SLA haplotype identification. Bone marrow samples were taken from each recipient for subsequent co-culture. In vitro culture and co-culture conditions were maintained and assessed. Histological analysis using hematoxylin and eosin staining was performed on the allograft that lasted the longest time showing the smallest macroscopic signs of rejection. A surgical protocol for Vascularized Composite Allograft (VCA) ear transplantation in a porcine biomodel was developed, including the skin. The presence of SLA-DRB1*01:02 and SLA-DRB1*06:01 haplotypes in the recipient and donor, respectively, showed concordance with positive crossmatch tests. In the allograft with the highest survival time, no histological signs of hyperacute rejection were found ten days after transplantation in the anastomosis area. The results obtained from this protocol can provide valuable recommendations for translational applications in face transplantation and regenerative medicine.