A study on compounds with spermicidal potential in <i>Calophyllum inophyllum </i>L. seed shell towards <i>Mus musculus</i>

Calophyllum inophyllum L is a plant found on the coast and is known to have pharmacological effects such as anti-oxidant, anti-cancer, anti-viral and anti-inflammatory. This study aimed to determine the ability of the ethanolic extract of the sampling seed shells as a spermicide for albino mice (Mus musculus), determine the growth inhibition of Candida albicans, and identify its compounds. The extraction of the C. inophyllum L seed shell was carried out by maceration. The quality of motility and abnormal morphology of mice sperms were observed under the microscope. The fungus inhibition assay was carried out by diffusion agar, and the identification of the active compounds by Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC/MS-MS). Sperm motility in ethanolic extract at the concentrations of 0.1, 0.3, and 0.5% were 10.85, 10.05, and 10.85%, respectively, and the abnormal morphology was 63.50, 65.62, and 72.67%, respectively. The partition results of the n-hexane extract showed that motility and abnormal morphology values at the concentrations of 0.1, 0.3, and 0.5% were 79.21 and 79.93%, and the abnormal morphology was 8.49, 4.30 and 0.00%, respectively. Fraction F6 at the concentration of 0.3% showed that its motility was 0.00% and abnormal morphology was 65.86%, so it did not provide inhibition against the C. albicans fungus. Fraction F6 contained compounds that had spermicidal potential were 2,5-Dioxo-1- pyrrolidinyl N-[(benzyloxy) carbonyl]-D-phenylalaninate, raubasine, and N-(4-{[2,4-Diamino-7,8-dyhydro-6 pteridinyl) methyl] (methyl) amino} benzoyl) L-glutamic acid or methotrexate through to cytotoxic effects.