METABOLIC RESEARCH OF GLYCOALKALOIDS BY TANDEM MASS SPECTROMETRY IN EIGHT VARIETIES OF COLORED POTATOES SOLANUM TUBEROSUM L.

The purpose of research is a metabolomic study of glycoalkaloids by tandem mass spectrometry in eight varieties of colored potatoes. In this work, extracts of colored potatoes, the breeding samples of which were obtained from the Siberian Federal Scientific Center for Agrobiotechnologies of the Russian Academy of Sciences, are studied for the presence of a variety of glycoalkaloids. Eight varieties were used: Tuleevskij, Kuznechanka, Pamyati Antoshkinoj, Tomichka, Gibrid 15/F-2-13, Gibrid 22103-10, Hy-brid 17-5/6-11, Sinilga. Glycoalkaloids are plant metabolites containing an oligosaccharide, a C27 steroid, and a heterocyclic nitrogen component. Initial screening by HPLC-MS showed that among the hundreds of compounds found in tubers, the composition of glycoalkaloids was particularly diverse. Potato glycoal-kaloids can be divided into two main classes: solanidane or spirosolan aglycones. It is believed that sola-nine and chaconine make up more than 90 % of the total glycoalkaloids of domesticated potatoes, with chaconine often more than solanine. Using tandem mass spectrometry (high-performance liquid extraction and BRUKER DALTONIKS ion trap), extracts of these varieties were analyzed for the presence of com-pounds from the glycoalkaloids family. The results of the study showed the presence of 22 glycoalkaloids in the proposed varieties, some of the identified glycoalkaloids have not yet been described in the world scientific literature.