THE EFFECT OF RHIZOMES AND ROOTS DECOCTION OF MEDICINAL HEMOPHLEBUS ON THE GROWTH OF CONDITIONALLY PATHOGENIC AND LACTIC ACID MICROORGANISMS IN VITRO

The aim of the study is to determine the sensitivity of representatives of conditionally pathogenic (causative agents of inflammatory and purulent-septic diseases of soft tissues, skin, mucous organs of digestion and reproduction of cattle in the Kemerovo Region) and normal intestinal microorganisms: S. aureus, E. coli, B. cereus , P. mirabilis, Lactobacillus spp., Bifidobacterium spp. to the extract of the roots and rhizomes of the burnet officinalis in various dilutions to establish the minimum inhibitory concentration of the prepared dosage form in the form of a solution. Conditionally pathogenic microorganisms are the main causative agents of inflammatory and purulent-septic diseases of soft tissues, skin, mucous organs of digestion and reproduction of cattle in the Kemerovo Region. The studies were carried out by the cultural method, with the addition of burnet extract, prepared according to the standard method, to nutritious meat-peptone agar. The experiment used solutions of burnet extract added to nutrient agar in the ratio 1:1, 1:2, 1:4, 1:8. Burnet extract in all dilutions inhibited the growth of S. aureus and representatives of normal microflora: Lactobacillus spp. and Bifidobacterium spp. E. coli showed insensitivity to the test extract at all dilutions. The growth of B. cereus and P. mirabilis was inhibited by the studied extract at a dilution of 1:1, 1:2, 1:4. At a dilution of 1:8, a good, distinct growth of colonies was observed that is typical of these types of microorganisms. It is effective to use burnet extract in the form of a solution in the treatment of purulent-septic processes of soft tissues and mucous membranes infected with S. aureus, as well as to prevent the development of an infectious process caused by it. The use of burnet extract for the treatment and prevention of infections caused by B. cereus and P. mirabilis may be ineffective due to the low inhibition of the growth of these microorganisms on dense nutrient media. Inhibition of the growth of normal microflora Lactobacillus spp. and Bifidobacterium spp. can disrupt the microecological balance of the digestive tract, so oral burnet extract is not recommended. It is necessary to confine to parenteral methods of using various dosage forms, including solutions containing burnet extract.