Evaluating the Role of KRAS and NRAS 3' Untranslated Region Polymorphisms in Susceptibility and Clinical Features of Laryngeal Squamous Cell Carcinoma

Laryngeal squamous cell cancer (LSCC) is one of the most common head and neck cancers in which genetic factors play an important role in its occurrence. This study investigated the association of <i>KRAS</i> and <i>NRAS</i> gene polymorphisms with the risk of LSCC. <i>KRAS</i> polymorphisms including rs712, rs61764370, rs8720, and rs9266, as well as NRAS rs14804, were compared in the patient group (n=120) and the control group (n=100). The Sanger sequencing method was used to identify these polymorphisms. The results showed that <i>KRAS</i> rs8720 is associated with an increased risk of LSCC; consequently, those with the CT genotype were at a higher risk than those with the CC genotype. Also, the CC genotype had a protective effect on rs14804 polymorphism of the <i>NRAS</i> gene. These findings show that some <i>KRAS</i> and <i>NRAS</i> polymorphisms can be used as diagnostic and prognostic biomarkers in LSCC, and their accurate identification by Sanger sequencing is of great importance in research related to cancer genetics.