AbNumPro: A comprehensive offline toolkit for antibody numbering and antigen-binding region prediction

Identifying complementary-determining regions (CDRs) and antigen-binding regions (ABRs) requires accurate antibody numbering, which is essential for therapeutic antibody development. AbNumPro is a comprehensive offline toolkit developed for antibody numbering and ABRs prediction, addressing the limitations of existing tools, which often lack comprehensiveness and rely solely on online services. By integrating five established numbering schemes—Kabat, Chothia, IMGT, Aho, and Martin—AbNumPro provides precise delineation of CDRs and ABRs, offering both compatibility with diverse research applications and the assurance of data security. • Identifying CDRs and ABRs requires accurate antibody numbering, which is essential for therapeutic antibody development. • AbNumPro integrates multiple numbering schemes and provides offline software to ensure data security. • AbNumPro is a pioneering software specifically designed to predict ABRs.