Distinct Urinary Metabolite Signatures Mirror In Vivo Oxidative Stress-Related Radiation Responses in Mice
Exposure to ionizing radiation disrupts metabolic pathways and causes oxidative stress, which can lead to organ damage. In this study, urinary metabolites from mice exposed to high-dose and low-dose whole-body irradiation (WBI HDR, WBI LDR) or partial-body irradiation (PBI BM2.5) were analyzed using targeted and untargeted metabolomics approaches. Significant metabolic changes particularly in oxidative stress pathways were observed on Day 2 post-radiation. By Day 30, the WBI HDR group showed persistent metabolic dysregulation, while the WBI LDR and PBI BM2.5 groups were similar to control mice. Machine learning models identified metabolites that were predictive of the type of radiation exposure with high accuracy, highlighting their potential use as biomarkers for radiation damage and oxidative stress.
Authors
- Amrita K. Cheema (ORCID: https://orcid.org/0000-0003-4877-7583)
- Dörthe Schaue (ORCID: https://orcid.org/0000-0002-5674-678X)
- Meth Jayatilake (ORCID: https://orcid.org/0000-0002-5780-9391)
- J. Hack (ORCID: https://orcid.org/0000-0003-2227-3846)
- Yaoxiang Li (ORCID: https://orcid.org/0000-0001-9200-1016)
- Shivani Bansal (ORCID: https://orcid.org/0000-0002-5095-5687)
- Mi-Heon Lee (ORCID: https://orcid.org/0000-0002-9938-0618)
- Marjan Boerma (ORCID: https://orcid.org/0000-0003-2249-5203)
- William Klotzbier
- Keisuke S. Iwamoto (ORCID: https://orcid.org/0000-0002-9290-4677)
- Baldev Singh
Institutions
- University of California, Los Angeles (US)
- Georgetown University (US)
- Georgetown University Medical Center (US)
- University of Arkansas for Medical Sciences (US)
Publication Details
- Journal
- Antioxidants
- Published
- 2024-12-27
- DOI
- https://doi.org/10.3390/antiox14010024
- Citations
- 2
- Primary Topic
- Effects of Radiation Exposure
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Georgetown University
- National Institutes of Health
- National Cancer Institute