Construction and characterization of bioluminescent <i>Salmonella</i> Reading outbreak and non-outbreak strains
<i>Salmonella enterica</i> serotype Reading has recently become a significant foodborne pathogen linked to poultry products. To enhance pathogen monitoring, this study developed bioluminescent strains of <i>S.</i> Reading by inserting the chloramphenicol acetyltransferase gene into a plasmid containing a bioluminescence gene cluster. These modified strains were transformed into outbreak and non-outbreak bacterial strains via electroporation. The bioluminescent strains demonstrated stable plasmid retention and high bioluminescence levels. They also showed growth comparable to their parent strains, even in the absence of antibiotics. These bioluminescent strains could potentially facilitate real-time monitoring and control of <i>S.</i> Reading in poultry industries.
Authors
- Reshma Ramachandran (ORCID: https://orcid.org/0000-0003-3845-3606)
- Anuraj T. Sukumaran (ORCID: https://orcid.org/0000-0002-7696-5947)
- K.S. Macklin (ORCID: https://orcid.org/0000-0002-2707-8866)
- Claudia D. Castañeda (ORCID: https://orcid.org/0000-0002-3381-1913)
- Hossam Abdelhamed (ORCID: https://orcid.org/0000-0003-2439-0659)
- Timothy Boltz (ORCID: https://orcid.org/0000-0002-6086-7744)
- A.S. Kiess
- Li Zhang (ORCID: https://orcid.org/0000-0002-3933-5794)
- Abubakar Shitu Isah
Institutions
- IDEXX Laboratories (France) (FR)
- Lakshmibai National Institute of Physical Education (IN)
- North Carolina State University (US)
- IDEXX Laboratories (United States) (US)
- Limagrain (France) (FR)
- Mississippi State University (US)
Publication Details
- Journal
- Microbiology Spectrum
- Published
- 2024-12-27
- DOI
- https://doi.org/10.1128/spectrum.01263-24
- Primary Topic
- bioluminescence and chemiluminescence research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- U.S. Department of Agriculture
- U.S. Poultry and Egg Association
- National Institute of Food and Agriculture