Antibiofilm Potential of Stevia Rebaudiana Bertoni Leaf Extract Irrigated Solution against The Bacteri Enterococcus Faecalis ATCC 29212

Disinfection of dental root canals 2.5% NaOCL is still a challenge related to its toxicity and the virulence of Enterococcus faecalis which is able to form biofilms in dental root canals. There is no evidence on the antibiofilm ability of Stevia rebaudiana Bertoni leaf extract against Enterococcus faecalis. Objective: To evaluate the efficacy of Stevia rebaudiana Bertoni leaf extract irrigant in preventing Enterococcus faecalis biofilm formation. Materials and Methods: This in vitro study utilized a Post-Test Only Control Group Design. Stevia rebaudiana Bertoni leaf extract irrigants were serially diluted to concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.56%. 2.5% NaOCl and Brain Heart Infusion-Broth (BHI-B) media with 2% sucrose were employed as the positive and negative controls, respectively. Enterococcus faecalis ATCC 29212 bacterial suspension was equated with Mc Farland 0.5 standard. Biofilm growth media using BHI-B + 2% sucrose. Antibiofilm potential using crystal violet assay. Measurement of optical density (OD) value at 490 nm. Results: Significant differences were identified using the Kruskal-Wallis test (p=0.000) between the mean experimental OD values. Mann-Whitney U test showed the mean experimental OD values of all Stevia rebaudiana Bertoni leaf extract irrigants were significantly smaller than the negative control (p<0.05). The 100% Stevia rebaudiana Bertoni leaf extract irrigants did not exhibit a significantly different mean optical density (OD) value compared to the positive control (p > 0.05), while 50%, 25%, 12.5%, 6.25%, 3.125% and 1.56% Stevia rebaudiana Bertoni leaf extract irrigants significantly outperformed the positive control (p<0.05). Conclusion: All irrigation solutions of Stevia rebaudiana Bertoni leaf extract proved to have antibiofilm potential of E.faecalis significantly greater than the negative control. The antibiofilm potential of 50%; 25%; 12.5%; 6.25%; 3.125% and irrigation solution containing 1.56% Stevia rebaudiana Bertoni leaf extract was significantly less than NaOCL 2.5% while 100% Stevia rebaudiana Bertoni leaf extract irrigant was not significantly different from NaOCL 2.5%.