Isolation and characterization of methicillin-resistant Staphylococcus aureus from bovine mastitis in North West Cameroon: public health implications
We confirmed MRSA in 69 of 201 (34.3%) S. aureus isolates recovered from a total of 300 samples. Of the 69 MRSA, 19 (27.5%) were from subclinical cases, while 50 (72.5%) were from clinical cases. The MRSA showed high resistance to penicillin (100%), ampicillin (100%), trimethoprim (69.6%), and tetracycline (69.6%) while susceptibility was observed for gentamicin (100%), vancomycin (95.7%), and ciprofloxacin (91.3%). Most isolates (65.2%, 45/69) were multidrug resistant. Thirteen antibiotypes (A<sub>1</sub>-A<sub>13</sub>) were identified and the most prevalent was A<sub>8</sub> (TMP<sup>R</sup>E<sup>R</sup>TET<sup>R</sup>AMP<sup>R</sup>P<sup>R)</sup>). All MRSA produced haemolysins, caseinase, and coagulase. Lipase, gelatinase and lecithinase were found in 97.1%, 94.2% and 91.3% of isolates respectively. Genotyping revealed coa (100%) and spa (68.1%) genes. We recommend educating dairy farmers on the public health implications of consuming unpasteurized raw milk and the implementation of proper hygiene practices in dairy farms.
Authors
- Lucy Ndip (ORCID: https://orcid.org/0000-0003-3511-6049)
- Roland N. Ndip (ORCID: https://orcid.org/0000-0003-0028-7024)
- Stella Smith (ORCID: https://orcid.org/0000-0003-2163-1189)
- Pascal Bessong (ORCID: https://orcid.org/0000-0003-0561-272X)
- Seraphine Nkie Esemu (ORCID: https://orcid.org/0000-0003-2497-4689)
- Nicoline F. Tanih
- Shantal Fuenbi Nya’Nying
Institutions
- South African Medical Research Council (ZA)
- University of Buea (CM)
- Center for Global Health (US)
- Nigerian Institute of Medical Research (NG)
- University of Virginia (US)
- University of Venda (ZA)
Publication Details
- Journal
- BMC Research Notes
- Published
- 2024-12-27
- DOI
- https://doi.org/10.1186/s13104-024-07073-3
- Citations
- 4
- Primary Topic
- Milk Quality and Mastitis in Dairy Cows
- Type
- article
- Field-Weighted Citation Impact
- 2.27