Determination of Uric Acid Using 2D-MoS2 Modified GCE

Abstract Uric acid is the end product of purine metabolism in the human body. The determination of the amount of uric acid in biological samples is made by many analytical methods. However, due to the high cost and time consumption of these methods, many sensors have been developed for the determination of uric acid by electrochemical methods. Because the determination of biological samples by electrochemical methods is possible in shorter times and with inexpensive devices. In this study, electrochemical determination of uric acid was performed by modifying the glassy carbon electrode with two-dimensional molybdenum disulfide using two different methods (drop-coating and electrochemical coating). From the uric acid determination with the MoS2(1)/GCE numbered electrode, the sensitivity was found to be 11.4 µA.mM-1, the linear operating range from 4 µM to 520 µM, and the detection limit was 0.8 µM. With MoS2(2)/GCE, the 1st linear working range against UA was found to be 0.1 µM – 20 µM, the sensitivity was 331 µA.mM-1 and the detection limit was 6.7x10-8 M. The 2nd linear operating range was 20 µM - 687 µM, and the sensitivity was determined as 62.4 µA.mM-1. In order to determine the efficiency of uric acid determination with MoS2(2)/GCE, UA determination in blood serum samples obtained from the hospital was successfully performed with MoS2(2)/GCE with a relative error of 3.7%.