Transgenic tomato strategies targeting whitefly eggs from apoplastic or ovary-directed proteins
mCherry proved an effective reporter for the desired tissue-specific expression in tomato, but insufficiently sensitive to allow for localization in feeding whiteflies or their eggs. Segregated homozygous transgenic tomato lines were important for drawing these conclusions. The implications of these observations to possible pest-control strategies including preliminary expression of analogous chitinase constructs are discussed.
Authors
- Michihito Deguchi (ORCID: https://orcid.org/0000-0001-8380-0000)
- Wayne R. Curtis (ORCID: https://orcid.org/0000-0001-6976-0890)
- Aisa Sam
- Sairam Rudrabhatla (ORCID: https://orcid.org/0000-0002-0663-7366)
- Samwel Muiruri (ORCID: https://orcid.org/0000-0003-1362-0447)
- Eva Mei Shouse (ORCID: https://orcid.org/0000-0002-7868-0742)
- David Krum (ORCID: https://orcid.org/0000-0003-2059-0653)
- Zachary Weston (ORCID: https://orcid.org/0009-0005-4577-5197)
- Michael Ream
- Yun-Ru Chen (ORCID: https://orcid.org/0000-0002-1672-8974)
- Natalie S. Thompson
- Aliya Fathima Anwar
Institutions
- Pennsylvania State University (US)
- Kenyatta University (KE)
- University of Georgia (US)
- Université Mohammed VI Polytechnique (MA)
Publication Details
- Journal
- BMC Plant Biology
- Published
- 2024-12-26
- DOI
- https://doi.org/10.1186/s12870-024-05852-5
- Primary Topic
- Plant Virus Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- Bill and Melinda Gates Foundation
- University of Pennsylvania
- Pennsylvania State University
- Defense Advanced Research Projects Agency
- Advanced Research Projects Agency
- Defense Sciences Office, DARPA