Reversine Induces Apoptosis of FaDu Hypopharyngeal Carcinoma Cells

Background: Hypopharyngeal carcinoma is an aggressive subtype of head and neck squamous cell carcinoma, characterized by poor prognosis and resistance to conventional therapies.Reversine, a 2,6-disubstituted purine derivative, exhibits anticancer properties by inducing apoptosis and inhibiting cell proliferation in various cancers.Methods: FaDu hypopharyngeal carcinoma cells were treated with reversine at concentrations of Control, 15 M, and 30 M. Cell viability was assessed using MTT and live/dead assays.Apoptotic features were analyzed by hematoxylin & eosin and DAPI staining, and DNA fragmentation assays.Immunoblotting was performed to evaluate caspase-3 and poly adenosine diphosphate-ribose polymerase (PARP) activation.Results: Reversine significantly reduced the viability of FaDu cells in a dose-dependent manner.Morphological changes, nuclear fragmentation, and DNA laddering confirmed apoptosis.Immunoblotting revealed increased levels of cleaved caspase-3 and PARP, indicating the activation of the intrinsic and extrinsic apoptotic pathways.Conclusion: Reversine effectively inhibited FaDu cell viability and induced apoptosis through intrinsic and extrinsic pathways, and represents a potential novel therapeutic agent for hypopharyngeal carcinoma.This study highlights a unique focus on subpharyngeal cancer, overcoming therapeutic resistance, and suggests its potential as an anticancer agent by targeting dual cell death pathways.