Relationship between Klotho gene methylation level and diet habit

Purpose: This study aimed to determine the relationship between the methylation level of the Klotho gene and nutritional habits. Materials and Methods: From our healthy sample group consisting of 20 people, two groups were created: 10 people fed with carbohydrates and 10 people had protein. Initially, a food consumption frequency determination form was administered as a survey to individuals. Based on the results of this survey, the amounts of food consumed by the participants (g/cc) were determined. According to the findings of the survey, two groups were formed: those classified as carbohydrate consumers (individuals consuming 33% or more of their diet from carbohydrates) and those classified as protein consumers (individuals consuming 17% or more of their diet from protein). Methylation level of Klotho gene in blood samples of individuals; DNA isolation, RT-PCR and Bisulfite Modification were examined. Results: In the carbohydrate diet group; there was a very strong and inverse correlation between fat and methylation percentages (r = -0.765, p = 0.05). There was a strong correlation between the percentages of carbohydrate and methylation (r = 0.778, p = 0.004). A strong correlation was also found between BMI and methylation percentage (r = 0.712, p = 0.01). There was a strong inverse correlation between cholesterol and methylation percentages (r =-0.556, p = 0.04). In the protein diet group, there was a strong inverse correlation between BMI and methylation (r =-0.635, p = 0.024). Conclusion: As a result of the analysis, the Klotho gene methylation percentage (33%) in individuals with a carbohydrate-based diet was found to be higher than that in individuals with a protein-based diet (17%). The data obtained indicate that as carbohydrate consumption increases, the methylation level of the Klotho gene also rises.