Synthesis and Characterization of Silver and Zinc Nanoparticles From <i>Vitex altissima</i> : Comparative Analysis of Anti‐Oxidant, Anti‐Inflammatory, Antibacterial, and Anti‐Biofilm Activities

Metal nanoparticles have attained much popularity due to their low toxicity, economic feasibility, and eco-friendly nature. The present study focuses on the synthesis of silver and zinc nanoparticles from Vitex altissima leaf extract, further characterized by UV/Vis spectral analysis, Powder-x-ray diffraction (XRD), FE-SEM, Fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta potential. Synthesized silver and zinc nanoparticles were screened for antioxidant, anti-inflammatory, antibacterial, and anti-biofilm activities. AgNPs exhibited moderate antioxidant activities compared to ZnNPs, which were studied using 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and ABTS assays. The anti-inflammatory effect was assessed using membrane stabilization and human red blood cell methods. Furthermore, both types of nanoparticles, AgNPs and ZnNPs, exhibited anti-biofilm activity against four MDR bacterial strains: Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Synthesized nanoparticles show antibacterial activity. Our data suggest that silver nanoparticles exhibited moderate activity compared to ZnNPs. These nanoparticles could act as potential antioxidant, anti-inflammatory, and antibacterial agents.