Biochemical Effects of Silver Nanoparticles Prepared by Chemical Reduction Method on Male Rat Kidney Functions and Antioxidant Defense Systems

Background: The present study was conducted at the Animal House Unit, Biotechnology Research Center, Al-Nahrain University, Iraq, Baghdad.This study aimed to synthesize and characterize silver nanoparticles (Ag NPs) and investigate the impact of varying concentrations of Ag NPs on biochemical parameters, antioxidant defense system, lipid peroxidation, Histology and Immunohistochemistry in Male Rat Kidney. Methods: There were fifty male rats (150–170 g) utilized. Five groups, each with ten rats, were created from the animals. Group II, III, IV and V received oral administration of Silver Nanoparticles (Ag NPs) at doses of 1/150 LD50, 1/100 LD50, 1/50 LD50 and 1/25 LD50 mg/kg BW/day for a duration of three weeks, with the first group serving as the control. At the end of the experiment, blood and kidney samples were collected to look at a number of factors. Result: Treatment with Silver Nanoparticles (Ag NPs) different concentrations reduced kidney content in comparison to control for glutathione S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx)and glutathione reductase (GR). When rats were treated with varying quantities of silver nanoparticles (Ag NPs), their protein levels were considerably lower than those of the control group.Treatment with various quantities of silver nanoparticles (Ag NPs) raised the urea and creatinine levels significantly, urea and creatinine concentration increases are considered to be significant markers of renal failure.