Progress in human diseases treatment CRISPR system

Clinical medicine and biological sciences make extensive use of the gene editing method symbolized by CRISPR-Cas9. With the rapid development of molecular biology technology in recent years, the CRISPR-Cas systemwhich stands for clustered regularly interspaced short palindromic repeatshas become a potent tool for gene editing. Due to its high efficiency, precision, and flexibility, it has surpassed last two generations in its application within the field of molecular biology. This article provides a detailed overview of the development history, working mechanism, and applications of this technology. The CRISPR-Cas system achieves specific site modifications by introducing insertions, deletions, or single base substitutions at particular genomic sites. It has made significant contributions to research and treatment in areas such as the therapies of tumors and genetic diseases. However, challenges such as off-target effects need to be addressed before its practical clinical utility can be rigorously validated through further research and clinical trials.