Determination of Antidiabetic Activity of Ethanolic Extract of Karas Tulang Leaves (Chloranthus Erectus) In Alloxan-Induced Wistar Rats

Diabetes mellitus is a chronic condition that affects the metabolism of carbohydrates (glucose) in the body. According to data from the 2018 Basic Health Research (Riskesdas), there was a 2.1% increase in the prevalence of diabetes mellitus in Indonesia. The objectives of this study are: 1) To determine the antidiabetic activity of ethanol extract from Karas Tulang leaves in Wistar strain rats modeled for diabetes mellitus induced by alloxan, and 2) To perform phytochemical screening of the ethanol extract of Karas Tulang leaves (Chloranthus erectus).The research method utilized an experimental design with four groups of Wistar strain white rats. The phytochemical screening conducted included tests for alkaloids, saponins, flavonoids, and tannins using the thin-layer chromatography (TLC) method. Based on the results of the phytochemical screening tests, it was found that Karas Tulang leaves contain alkaloids, saponins, flavonoids, and tannins. The effectiveness of the antidiabetic activity was analyzed using SPSS through statistical tests, including normality, homogeneity, Kruskal-Wallis, and Mann-Whitney tests. Based on the statistical test results, it was concluded that the ethanol extract of Karas Tulang leaves at doses of 50 and 100 mg/kgBW is effective in reducing blood glucose levels.