Incidence of Helicobacter pylori in Type l Diabetes Mellitus Patients and Association with Certain HLA Alleles

Abstract Background: Helicobacter pylori infection was mainly the factor that causes both gastric tumors and lymphomas. Objectives: This study aimed to detect H. pylori infection and investigate any relationship between the infection of H. pylori and the disease of diabetes, to find out if there is an association with certain human leukocyte antigen (HLA), and to study the frequency of H. pylori infection among type l diabetic and nondiabetic patients. Materials and Methods: Several 140 patients diagnosed with type 1 diabetes mellitus (T1DM), as well as 60 healthy individuals (nondiabetic) were enrolled in this study. The oligonucleotide technique of sequence-specific was performed for genotyping of HLAs using a polymerase chain reaction machine. A specific screening ( H. pylori antibodies combo rapid test) is used to diagnose H. pylori infection by detection of specific antibodies in the samples. Results: This study revealed that people who are diagnosed T1DM are more predisposed to be infected by H. pylori (61%) compared with healthier people, and the pathogen infects significantly females compared with males. Results also revealed a great frequency of DQB1*0101 allele within diabetics (30%) in comparison with healthy (control) group, whereas HLA-A*3301,*1122 B*0826,*3948, DRB1*0701,*1101, and HLA-DQB1-*0604,*630,*730 alleles were recorded noteworthy low frequency according to the results after statistical analysis of P value ( P < 0.05) in patients compared with healthy control groups. Conclusion: Diabetic women of the same age are more susceptible to the infection compared with males. The great frequencies of HLA-DQB1*0101 alleles could be a risk factor for T1DM patients.