A Molecular Logic Gate Enables Unconventional Super-resolution Same-Day Imaging of Lysosome Membrane in Live Cells
Lysosomes are acidic membrane-bound organelles that aid digestion, excretion, and cell renewal. The lysosomal membranes are essential for maintaining lysosomal functions and cellular homeostasis. In this work, we developed a molecular "NOR" logic gate, <b>SIATFluor-580L</b>, by introducing malachite green into the spirocyclic rhodamine. <b>SIATFluor-580L</b> requires restriction of molecular rotation of the malachite green motif (<b>Input 1</b>, tight membrane structure) and a large amount of H<sup>+</sup> ions to convert the spirocyclic rhodamine into the zwitterionic form (<b>Input 2</b>, acidic environment) to produce a fluorescent product (<b>Output</b>), providing a fluorogenic probe to visualize the lysosomal membrane dynamics in living cells with subdiffraction resolution by using HyVolution (also known as Lightning), an unconventional and inexpensive super-resolution imaging approach based on a basic confocal optical system.
Authors
- Yi Zhou (ORCID: https://orcid.org/0000-0002-4176-5793)
- Lintao Cai (ORCID: https://orcid.org/0000-0002-2461-6390)
- Fufeng Liu (ORCID: https://orcid.org/0000-0002-9454-2382)
- Yangzi Xie
- Wen Li (ORCID: https://orcid.org/0000-0001-6783-1464)
- Pengfei Zhang (ORCID: https://orcid.org/0000-0003-0390-3806)
- Fan Pan (ORCID: https://orcid.org/0009-0008-6325-0089)
- Zhe Jiao (ORCID: https://orcid.org/0000-0002-4600-8927)
- Yongfan Men (ORCID: https://orcid.org/0000-0001-8265-1787)
- Yuan Luo (ORCID: https://orcid.org/0000-0001-9996-7817)
Institutions
- Tianjin University of Science and Technology (CN)
- Chinese Academy of Sciences (CN)
- Dongguan University of Technology (CN)
- Laboratoire d’Imagerie Biomédicale (FR)
- Institute for Biomedicine (IT)
- Shenzhen Luohu People's Hospital (CN)
- Shenzhen Institutes of Advanced Technology (CN)
- Imperial College London (GB)
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2024-12-25
- DOI
- https://doi.org/10.1021/acs.analchem.4c05907
- Citations
- 2
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 1.32
Funders
- Science and Technology Foundation of Shenzhen City
- National Natural Science Foundation of China
- Chinese Academy of Sciences
- National Key Research and Development Program of China
- Special Project for Research and Development in Key areas of Guangdong Province