An interkinetic envelope surrounds chromosomes between meiosis I and II in <i>C. elegans</i> oocytes
At the end of cell division, the nuclear envelope reassembles around the decondensing chromosomes. Female meiosis culminates in two consecutive cell divisions of the oocyte, meiosis I and II, which are separated by a brief transition phase known as interkinesis. Due to the absence of chromosome decondensation and the suppression of genome replication during interkinesis, it has been widely assumed that the nuclear envelope does not reassemble between meiosis I and II. By analyzing interkinesis in C. elegans oocytes, we instead show that an atypical structure made of two lipid bilayers, which we termed the interkinetic envelope, surrounds the surface of the segregating chromosomes. The interkinetic envelope shares common features with the nuclear envelope but also exhibits specific characteristics that distinguish it, including its lack of continuity with the endoplasmic reticulum, unique protein composition, assembly mechanism, and function in chromosome segregation. These distinct attributes collectively define the interkinetic envelope as a unique and specialized structure that has been previously overlooked.
Authors
- Julien Dumont (ORCID: https://orcid.org/0000-0001-5312-9770)
- Rémi Le Borgne (ORCID: https://orcid.org/0000-0002-5389-9721)
- Lionel Pintard (ORCID: https://orcid.org/0000-0003-0286-4630)
- Peter Askjaer (ORCID: https://orcid.org/0000-0003-3192-4428)
- Julie C. Canman (ORCID: https://orcid.org/0000-0001-8135-2072)
- Laura Bellutti (ORCID: https://orcid.org/0000-0001-9886-9430)
- Jean‐Marc Verbavatz (ORCID: https://orcid.org/0000-0002-4654-1174)
- Layla El Mossadeq (ORCID: https://orcid.org/0009-0007-2786-3673)
Institutions
- Centre National de la Recherche Scientifique (FR)
- Université Paris Cité (FR)
- Centro Andaluz de Biología del Desarrollo (ES)
- Institut Jacques Monod (FR)
- Columbia University (US)
- Universidad Pablo de Olavide (ES)
Publication Details
- Journal
- The Journal of Cell Biology
- Published
- 2024-12-26
- DOI
- https://doi.org/10.1083/jcb.202403125
- Citations
- 3
- Primary Topic
- Nuclear Structure and Function
- Type
- article
- Field-Weighted Citation Impact
- 0.70
Funders
- Infrastructures en Biologie Santé et Agronomie
- Agence Nationale de la Recherche
- Institut National de la Santé et de la Recherche Médicale
- Ligue Contre le Cancer
- Centre National de la Recherche Scientifique
- Fondation Bettencourt Schueller
- National Institutes of Health
- School of Medicine and Public Health, University of Wisconsin-Madison
- European Research Council
- European Regional Development Fund
- Agencia Estatal de Investigación