<i>Aristolochia</i> of <i>Siphisia</i> section <i>in vitro</i>

The results of the study represent an optimization protocol for clonal micropropagation of 4 species of the genus Aristolochia, Siphisia section. Plants of these species are rare, endemic and medicinal plants. 6-Benzylaminopurine (BAP) (0.5, 1.0, 1.2 and 1.4 mg/L) and indolylbutyric acid (IBA) (0.5 and 1.0 mg/L) were used in this work. The use of subcultivation with different nutrient media (MS supplemented with 0.5 mg/l BAP at the propagation stage and ½ WPM with the addition of 0.5 mg/l IBA at the rooting stage) contributes to the mass production of microplants. The maximum values of the microshoot height for each species were detected on the medium with 0.5 mg/l BAP. Explants of A. macrophylla and A. manshuriensis were characterized with the greatest reproduction coefficient (34 pcs.); while A. californica explants displayed the least coefficient (18.6 pcs.). The difference is associated with the activation of a different number of buds in each species.