Bioanalysis of protein-unbound prednisolone in serum using equilibrium dialysis followed by liquid chromatography-tandem mass spectrometry
An LC-MS/MS assay for prednisolone in serum was developed and validated, with a successful equilibrium dialysis technique to obtain protein-unbound prednisolone prior to quantification. This assay is considered suitable for pharmacokinetic studies.
Authors
- Matthijs van Luin (ORCID: https://orcid.org/0000-0001-8409-9316)
- Alwin D. R. Huitema (ORCID: https://orcid.org/0000-0003-1939-4639)
- Eef G.W.M. Lentjes (ORCID: https://orcid.org/0000-0003-4055-2291)
- Julia E. Möhlmann (ORCID: https://orcid.org/0000-0001-6496-937X)
- Arjen M. Punt (ORCID: https://orcid.org/0000-0003-3336-1980)
Institutions
- Utrecht University (NL)
- University Medical Center Utrecht (NL)
- Princess Máxima Center (NL)
Publication Details
- Journal
- Journal of Chromatography B
- Published
- 2024-12-27
- DOI
- https://doi.org/10.1016/j.jchromb.2024.124440
- Citations
- 1
- Primary Topic
- Adrenal Hormones and Disorders
- Type
- article
- Field-Weighted Citation Impact
- 0.36