In vivo: Anti-larvicidal (anthelmintic) activity of Spondias pinnata against Fasciola gigantica larvae

The aim of the present study is to evaluate in vivo anti-larvicidal (anthelmintic) activities of Spondias pinnata plant against sporocyst, redia, and cercaria (Fasciola gigantica) larvae. The liver fluke species of F. gigantica are caused zoonotic diseases in cattle and human populations. The common intermediate host snail Lymnaeids (Lymnaea acuminata) are carriers of the Fasciola. It can be controlled by the break of the life cycle in the intermediate host snail. The in vivo exposure of leaf powder and, various organic extracts, of S. pinnata, was observed up to 8h at various concentrations against sporocyst, redia, and cercaria. The anti-larvicidal effect of different preparations was observed at 2, 4, 6, and 8h of the exposure period. The exposure of leaf powder of S. pinnata in vivo was more effective against the sporocyst (2h LC50 68.64mg/L and 8h LC50 72.13mg/L). The column extract of leaf powder (CELP) of S. pinnata against sporocyst, redia, and cercaria in 2h LC50 was 66.38, 69.63, and 68.33 mg/L and 8h LC50 was 63.10, 66.03, and 65.86 mg/L, respectively. The ethanolic column extract of the S. pinnata leaf powder have better anti-larvicidal activities than the other organic extract.