The characterization of outer membrane vesicles (OMVs) and their role in mediating antibiotic-resistance gene transfer through natural transformation in Riemerella anatipestifer
Riemerella anatipestifer (R. anatipestifer, RA) is the etiological agent of duck serositis, an acute multisystemic disease in ducks that is globally distributed and causes serious economic losses in the duck industry. Despite exhibiting multidrug resistance, the transmission mechanism of its antibiotic resistance genes (ARGs) remains incompletely identified. To contribute to addressing this gap, in this study, outer membrane vesicles (OMVs) from the RA strain CH-1 were isolated and characterized to investigate their involvement in ARG transfer in RA. Sequencing and data analysis revealed that RA CH-1 OMVs had ∼2.04 Mb genomic size, representing 88.3 % of the RA CH-1 genomic length. Proteomic analysis showed that OMVs contained 577 proteins, representing 27.2 % of the bacterial proteins. Subsequent investigations demonstrated that OMVs from antibiotic-resistant strains transferred ARG fragments and plasmids to the sensitive strain RA ATCC11845, relying on the natural transformation system, and the transformants exhibited corresponding resistance. Overall, OMV-mediated horizontal transfer of ARGs serving as a significant mechanism for acquiring multiple resistance genes in R. anatipestifer.
Authors
- Juan Huang (ORCID: https://orcid.org/0000-0001-7129-602X)
- Yizhou Yao (ORCID: https://orcid.org/0000-0001-7976-1133)
- Qiao Yang (ORCID: https://orcid.org/0000-0001-7401-2168)
- Mafeng Liu (ORCID: https://orcid.org/0000-0002-6834-3128)
- Yuhao Yang (ORCID: https://orcid.org/0000-0002-2543-8450)
- Mengying Wang (ORCID: https://orcid.org/0000-0002-2609-9454)
- Zhen Wu (ORCID: https://orcid.org/0000-0003-1311-5627)
- Xumin Ou (ORCID: https://orcid.org/0000-0003-0456-6362)
- Renyong Jia (ORCID: https://orcid.org/0000-0002-3539-9184)
- Dekang Zhu (ORCID: https://orcid.org/0000-0002-7314-1088)
- Shaqiu Zhang
- Di Sun (ORCID: https://orcid.org/0000-0001-9980-5439)
- Mingshu Wang (ORCID: https://orcid.org/0000-0001-5260-3143)
- Ling Zhang (ORCID: https://orcid.org/0000-0002-8369-3927)
- Yu He
- Shun Chen (ORCID: https://orcid.org/0000-0002-7488-1037)
- Ying Wu (ORCID: https://orcid.org/0009-0008-1808-3349)
- Bin Tian (ORCID: https://orcid.org/0000-0001-8903-8256)
- Anchun Cheng
- Yanling Yu
- Xinxin Zhao
Institutions
- China Animal Disease Control Center (CN)
- Chengdu Center for Disease Control and Prevention (CN)
- Sichuan Center for Disease Control and Prevention (CN)
Publication Details
- Journal
- Poultry Science
- Published
- 2024-12-26
- DOI
- https://doi.org/10.1016/j.psj.2024.104730
- Citations
- 5
- Primary Topic
- Bacterial Infections and Vaccines
- Type
- article
- Field-Weighted Citation Impact
- 1.72
Funders
- National Natural Science Foundation of China
- China Agricultural Research System
- Natural Science Foundation of Sichuan Province