Trifunctional lipid derivatives: PE's mitochondrial interactome
Phosphatidylethanolamine (PE) is a ubiquitous lipid species in higher eukaryotes. Here, we synthesized a multifunctionalized PE derivative (1) designed to identify PE-binding proteins in intact cells through photo-crosslinking and subsequent isolation and proteomic analysis of the PE-protein conjugates. We show that the tool is also useful for tracking PE translocation to mitochondria after uncaging. A trifunctional phosphatidic acid derivative was rapidly metabolized and is of more limited use.
Authors
- Scotland Farley (ORCID: https://orcid.org/0000-0001-5761-700X)
- Ana Kojic (ORCID: https://orcid.org/0000-0002-1829-9990)
- Rainer Mueller (ORCID: https://orcid.org/0000-0003-3464-494X)
- Frank Stein (ORCID: https://orcid.org/0000-0001-9695-1692)
- Carsten Schultz (ORCID: https://orcid.org/0000-0002-5824-2171)
- Per Haberkant (ORCID: https://orcid.org/0000-0002-5608-9062)
- Alix Thomas
Institutions
- Oregon Health & Science University (US)
- European Molecular Biology Laboratory (DE)
Publication Details
- Journal
- Chemical Communications
- Published
- 2024-12-26
- DOI
- https://doi.org/10.1039/d4cc03599b
- Citations
- 4
- Primary Topic
- Mitochondrial Function and Pathology
- Type
- article
- Field-Weighted Citation Impact
- 0.94
Funders
- National Institutes of Health