Comparison of qPCR protocols for quantification of “Candidatus Saccharibacteria”, belonging to the Candidate Phyla Radiation, suggests that 23S rRNA is a better target than 16S rRNA
Overall, our results show that the 23S-based qPCR protocol is more precise than the 16S-based ones in quantifying "Ca. Saccharibacteria", although all protocols probably underestimate specific lineages. These results underline the current limits in quantifying "Ca. Saccharibacteria", highlighting the needs for novel experimental strategies or methods. Indeed, the underestimation of "Ca. Saccharibacteria" in clinical samples could hide its role in human health and in the development of immune-mediated diseases.
Authors
- Ella Pagliarini (ORCID: https://orcid.org/0000-0001-5261-9276)
- Stella Papaleo (ORCID: https://orcid.org/0000-0002-6277-9502)
- Riccardo Nodari (ORCID: https://orcid.org/0000-0002-3485-2485)
- Simona Panelli (ORCID: https://orcid.org/0000-0001-6083-8786)
- Enza D’Auria (ORCID: https://orcid.org/0000-0003-2750-5810)
- Gianvincenzo Zuccotti (ORCID: https://orcid.org/0000-0002-2795-9874)
- Francesco Comandatore (ORCID: https://orcid.org/0000-0001-8298-4792)
- Camilla Cattaneo (ORCID: https://orcid.org/0000-0002-3671-4334)
- Lodovico Sterzi (ORCID: https://orcid.org/0009-0000-3153-0977)
- Giorgia Bettoni
- Clara Bonaiti
Institutions
- University of Milan (IT)
Publication Details
- Journal
- PLoS ONE
- Published
- 2024-12-26
- DOI
- https://doi.org/10.1371/journal.pone.0310675
- Citations
- 2
- Primary Topic
- Cleft Lip and Palate Research
- Type
- article
- Field-Weighted Citation Impact
- 1.10
Funders
- Università degli Studi di Milano