Spatiotemporal single-cell roadmap of human skin wound healing
Wound healing is vital for human health, yet the details of cellular dynamics and coordination in human wound repair remain largely unexplored. To address this, we conducted single-cell multi-omics analyses on human skin wound tissues through inflammation, proliferation, and remodeling phases of wound repair from the same individuals, monitoring the cellular and molecular dynamics of human skin wound healing at an unprecedented spatiotemporal resolution. This singular roadmap reveals the cellular architecture of the wound margin and identifies FOSL1 as a critical driver of re-epithelialization. It shows that pro-inflammatory macrophages and fibroblasts sequentially support keratinocyte migration like a relay race across different healing stages. Comparison with single-cell data from venous and diabetic foot ulcers uncovers a link between failed keratinocyte migration and impaired inflammatory response in chronic wounds. Additionally, comparing human and mouse acute wound transcriptomes underscores the indispensable value of this roadmap in bridging basic research with clinical innovations.
Authors
- Yunting Xiao
- Xiaowei Bian (ORCID: https://orcid.org/0000-0001-8999-7078)
- Wenjun He (ORCID: https://orcid.org/0000-0003-2043-1329)
- Daniela Basurto-Lozada (ORCID: https://orcid.org/0000-0003-3943-8424)
- Chunyan Cao (ORCID: https://orcid.org/0000-0001-8324-8199)
- Nusayhah Hudaa Gopee (ORCID: https://orcid.org/0000-0001-8507-5682)
- Maria Kasper (ORCID: https://orcid.org/0000-0002-6117-2717)
- Clare L. Bennett (ORCID: https://orcid.org/0000-0001-9146-2347)
- Pehr Sommar (ORCID: https://orcid.org/0000-0002-9789-9221)
- Lihua Luo
- Lei Ming Guo (ORCID: https://orcid.org/0000-0002-6072-2377)
- Yongjian Chen (ORCID: https://orcid.org/0000-0002-2930-8450)
- Muzlifah A. Haniffa (ORCID: https://orcid.org/0000-0002-3927-2084)
- Åsa K. Björklund (ORCID: https://orcid.org/0000-0003-2224-7090)
- Jiating Wang
- Karl Annusver (ORCID: https://orcid.org/0000-0002-9515-7216)
- Dave Horsfall (ORCID: https://orcid.org/0000-0002-8086-812X)
- Li Li (ORCID: https://orcid.org/0000-0002-7898-6100)
- Zhuang Liu (ORCID: https://orcid.org/0000-0001-8938-0086)
- Dongqing Li (ORCID: https://orcid.org/0000-0003-0588-9390)
- Liping Zhu (ORCID: https://orcid.org/0000-0003-4039-4190)
- Letian Zhang (ORCID: https://orcid.org/0000-0002-0987-0905)
- Ning Xu (ORCID: https://orcid.org/0000-0003-4868-3798)
- Juan Gao
Institutions
- Karolinska University Hospital (SE)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Karolinska Institutet (SE)
- Soochow University (CN)
- Chinese Academy of Medical Sciences Dermatology Hospital (CN)
- First Affiliated Hospital of Soochow University (CN)
- University College London (GB)
- Newcastle University (GB)
Publication Details
- Journal
- Cell stem cell
- Published
- 2024-12-26
- DOI
- https://doi.org/10.1016/j.stem.2024.11.013
- Citations
- 110
- Primary Topic
- Single-cell and spatial transcriptomics
- Type
- article
- Field-Weighted Citation Impact
- 25.72
Funders
- Medical Research Council
- Biotechnology and Biological Sciences Research Council