Extraction of bioactive compounds from Lactobacillus gasseri and its food applications

Our project involves extracts derived from L.gasseri, characterization of secondary metabolites using UV visible spectroscopy and thin layer chromatography. Antimicrobial activity of the extracted metabolites are determined using well diffusion method and the results indicates that these secondary metabolites have the potential to inhibit the growth of gram positive bacteria such as Staphylococcus aures and Bacillus cerus and gram negative bacteria such as Shigella dysenteriae and Salmonella typhi. Antifungal activity was performed against Aspergillus oryzae and Aspergillus flavus and the results indicate that these extracted secondary metabolites doesn’t have antifungal property. MIC was determined against the pathogen using broth dilution method. Moreover, the applications of extracted secondary metabolites in improving the shelf life of the fruits are determined. Plum fruit and Avacado fruits are taken into study for our project and results indicates that coating of secondary metabolites over the fruits improve the shelf life.