Analysis of polymorphism of DNA microsatellite loci of false flax Camelina sativa

False flax (Camelina sativa) is a dicotyledonous plant of the Brassicaceae family, native to southern Europe and south-west Asia. False flax requires less water and fertilizers and, unlike other crops, is resistant to pests and pathogens. False flax seed oil contains unsaturated fatty acids: linolenic, linoleic, oleic and eicosenoic acids, with a low content of erucic acid. It is used in the production of fodder, biodiesel, polymers, varnishes, paint oil and paints, in cosmetology and the food industry. For effective development of new varieties of false flax, it is necessary to study its genetic diversity using molecular genetic methods, including SSR-markers. Microsatellites are widely used in plant genome analysis. The aim of this work was to study the genetic diversity of winter and spring forms of false flax bred at V.S. Pustovoit All-Russian Research Institute of Oil Crops (VNIIMK) using new experimental SSR-markers. In this work, we studied 18 winter and spring varieties of winter and spring false flax from the VNIIMK collection and two samples of the variety Krepysh. Genetic diversity was studied using nine SSR-markers. PCR products were detected by capillary electrophoresis on a Nanofor-05 genetic analyzer. As a result, 42 alleles were detected, ranging from two to seven alleles per locus. The average effective number of alleles was 3.00. The average polymorphic information content (PIC) index was 0.66. Out of 20 genotypes, eight genotypes could not be distinguished by nine SSR-markers. To identify them, the number of SSR loci should be increased. Samples of the variety Krepysh differ in seven loci. The study showed genetic heterogeneity of four varieties of false flax. The study of intravarietal polymorphism in the false flax varieties bred at VNIIMK will be continued.