Characterization of the protein complex of soybean varieties bred at V.S. Pustovoit All-Russian Research Institute of Oil Crops

The biochemical composition of soybean seeds determines the use of soybeans as a source of food and feed proteins. In this study, the protein complex of seeds of soybean varieties bred at V.S. Pustovoit All-Russian Research Institute of Oil Crops was evaluat-ed. The mass fraction of protein was determined by the Kjeldahl method on a VELP Scientifica device (Italy). Amino-acid composition was determined by high-performance liquid chromatography on a Sevco ARM-1000 amino-acid analyzer (Russia). Standard methods were used in the work. The ratio of amino-acid composition of soybean protein by functional groups as well as the protein content in soybean varieties bred at V.S. Pustovoit All-Russian Research Institute of Oil Crops (39.35–42.93%) were determined. The wholesomeness of soybean protein was estimated on the basis of the content of essential amino ac-ids in relation to the FAO/WHO reference protein. During the research it was determined that all essential amino acids have score more than 100%. The biological value of the soybean samples was calculated and ranged from 71.20 to 79.10 %. The ratio of differences in amino acid composition varied from 20.90 to 28.80%. The utility coefficient of the amino acid composition of soybean seed protein differed slightly among varieties (0.68-0.72 fractions of unit). The best ratio of qualitative characteristics of the protein complex was observed in the variety Puma.

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Publication Details

Journal
Oil Crops
Published
2024-12-25
DOI
https://doi.org/10.25230/2412-608x-2024-4-200-39-44
Primary Topic
Soybean genetics and cultivation
Type
article
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article

Characterization of the protein complex of soybean varieties bred at V.S. Pustovoit All-Russian Research Institute of Oil Crops

С.Г. Ефименко, Yu.M. Serova
Oil Crops
Soybean genetics and cultivation
article

Characterization of the protein complex of soybean varieties bred at V.S. Pustovoit All-Russian Research Institute of Oil Crops

С.Г. Ефименко, Yu.M. Serova
article en

Abstract

The biochemical composition of soybean seeds determines the use of soybeans as a source of food and feed proteins. In this study, the protein complex of seeds of soybean varieties bred at V.S. Pustovoit All-Russian Research Institute of Oil Crops was evaluat-ed. The mass fraction of protein was determined by the Kjeldahl method on a VELP Scientifica device (Italy). Amino-acid composition was determined by high-performance liquid chromatography on a Sevco ARM-1000 amino-acid analyzer (Russia). Standard methods were used in the work. The ratio of amino-acid composition of soybean protein by functional groups as well as the protein content in soybean varieties bred at V.S. Pustovoit All-Russian Research Institute of Oil Crops (39.35–42.93%) were determined. The wholesomeness of soybean protein was estimated on the basis of the content of essential amino ac-ids in relation to the FAO/WHO reference protein. During the research it was determined that all essential amino acids have score more than 100%. The biological value of the soybean samples was calculated and ranged from 71.20 to 79.10 %. The ratio of differences in amino acid composition varied from 20.90 to 28.80%. The utility coefficient of the amino acid composition of soybean seed protein differed slightly among varieties (0.68-0.72 fractions of unit). The best ratio of qualitative characteristics of the protein complex was observed in the variety Puma.

Oil CropsVol. 4(200)
Zero hunger
Openalex Percentile: Top 86%
Soybean genetics and cultivation
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