Efficient, broadly tunable, hollow-fiber source of megawatt pulses for multiphoton microscopy

Three-photon fluorescence microscopy (3PM) has driven rapid progress in deep-tissue imaging beyond the depth limit of two-photon microscopy, with impacts in neuroscience, immunology, and cancer biology. Three-photon excitation places a premium on ultrashort pulses with high peak power in the 1300- and 1700-nm wavelength bands, which allow deepest imaging. The inefficiency and cost of current sources of these pulses present major barriers to the use of 3PM in biomedical research labs. Fiber sources of such pulses could potentially alleviate these problems, but the peak-power limitations of optical fibers have limited their use in 3PM. Here, we describe a fiber-based source of femtosecond pulses with multi-megawatt peak power. Femtosecond pulses at 1030 nm are launched into an antiresonant hollow-core fiber filled with argon. By varying only the gas pressure, pulses with hundreds of nanojoules of energy and sub-100 fs duration are obtained at wavelengths between 850 and 1700 nm. This approach is a new route to an efficient and potentially low-cost source for deep-tissue imaging. In particular, 960-nJ and 50-fs pulses are generated at 1300 nm with a conversion efficiency of 10%. The nearly 20-MW peak power is an order of magnitude higher than the previous best from a femtosecond solid-core fiber source at 1300 nm. As an example of the capabilities of the source, these pulses are used to image structure and neuronal activity in a mouse brain as deep as 1.1 mm below the dura.