Determination of the genetic dimension of Pseudomonas stutzeri which of nitrogen-fixation bacteria in a random PCR method

Bacteria play an important role in fixing atmospheric nitrogen and converting it into compounds that can be used by plants. Due to the importance of this topic, this type of bacteria was chosen, and its genetic dimension was studied. The nitrogen-fixing strain of Pseudomonas stutzeri, or those that have already been positively identified, should be collected from the wild or from laboratory cultures. Pseudomonas stutzeri strain was collected and their genomic DNA was extracted. This can be done using regular methods for extracting DNA. The purpose of this research is to generate a diverse panel of PCR amplification primers. Primers chosen at random are short sequences of nucleotides that can bind to various locations in the bacterial genome. These primers can be used to initiate polymerase chain reaction (RPCR) amplification at a high number of random positions within the DNA, allowing researchers to probe a wide range of genes. Fifteen different bundles, ranging in size from 200 to 3000 base pairs, were recorded. Thirteen differential bundles of 350, 425, 600, 650, 700, 800, 950, 1000, 1300, 1400, 1500, 1700, and 3000 base pairs are featured by the inclusion of two public bundles of 200 and 2000. Fifteen different bundles, ranging in size from 200 to 3000 base pairs, were recorded. Thirteen differential bundles of 350, 425, 600, 650, 700, 800, 950, 1000, 1300, 1400, 1500, 1700, and 3000 base pairs are featured by the inclusion of two public bundles of 200 and 2000.

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Publication Details

Journal
Journal of Wasit for Science and Medicine
Published
2024-12-25
DOI
https://doi.org/10.31185/jwsm.511
Primary Topic
Genomics and Phylogenetic Studies
Type
article
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article

Determination of the genetic dimension of Pseudomonas stutzeri which of nitrogen-fixation bacteria in a random PCR method

Fawz Abdul Al-Salam Kalil Al-Saffar
Journal of Wasit for Science and Medicine
Genomics and Phylogenetic Studies
article

Determination of the genetic dimension of Pseudomonas stutzeri which of nitrogen-fixation bacteria in a random PCR method

Fawz Abdul Al-Salam Kalil Al-Saffar
article en

Abstract

Bacteria play an important role in fixing atmospheric nitrogen and converting it into compounds that can be used by plants. Due to the importance of this topic, this type of bacteria was chosen, and its genetic dimension was studied. The nitrogen-fixing strain of Pseudomonas stutzeri, or those that have already been positively identified, should be collected from the wild or from laboratory cultures. Pseudomonas stutzeri strain was collected and their genomic DNA was extracted. This can be done using regular methods for extracting DNA. The purpose of this research is to generate a diverse panel of PCR amplification primers. Primers chosen at random are short sequences of nucleotides that can bind to various locations in the bacterial genome. These primers can be used to initiate polymerase chain reaction (RPCR) amplification at a high number of random positions within the DNA, allowing researchers to probe a wide range of genes. Fifteen different bundles, ranging in size from 200 to 3000 base pairs, were recorded. Thirteen differential bundles of 350, 425, 600, 650, 700, 800, 950, 1000, 1300, 1400, 1500, 1700, and 3000 base pairs are featured by the inclusion of two public bundles of 200 and 2000. Fifteen different bundles, ranging in size from 200 to 3000 base pairs, were recorded. Thirteen differential bundles of 350, 425, 600, 650, 700, 800, 950, 1000, 1300, 1400, 1500, 1700, and 3000 base pairs are featured by the inclusion of two public bundles of 200 and 2000.

Journal of Wasit for Science and MedicineVol. 17(4)
Openalex Percentile: Top 79%
Genomics and Phylogenetic Studies
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