Characterization of an Acetogenin-Carrying Nanosuspension and Its Effect on Bacteria of Interest in the Poultry Industry

This work aimed to develop a nanosuspension (NSps) as an acetogenin (ACGs) carrier, using soy lecithin (SL) and hydroxypropyl-β-cyclodextrin (βCD) named NSps-βCDSL-ACGs. It was characterized by various spectroscopic techniques (DLS, FTIR, UV-vis diffuse reflectance). Moreover, the NSps morphology was observed by transmission electron microscopy (TEM). Also, the antibacterial activity of NSps-βCDSL-ACGs was evaluated against strains of interest in the poultry sector. NSps-βCDSL-ACGs presented nanometric size (207-239 nm), acceptable polydispersity index (PDI) values (0.13-0.17) and a high Z potential value (-47.17-50.36 mV), demonstrating high stability. The presence of ACGs in NSps-βCDSL-ACGs was confirmed by FTIR analysis. The nanoparticles had a spherical shape and exhibited high inhibition potential against <i>Salmonella</i> Enteritidis (88.18%), <i>Streptococcus gallolyticus</i> (88.01%), <i>Salmonella</i> Typhimurium (86.28%) and <i>Salmonella</i> Infantis (77.02%) strains up to 48 h, and a reduction of up to 3 log CFU/mL was achieved for <i>S.</i> Typhimurium. Therefore, NSps-βCDSL-ACGs is an attractive option for implementing ACGs administration in the poultry sector to reduce the use of antibiotics and minimize bacterial resistance.