A Two-Step Protocol for Isolation and Maintenance of Lung Cancer Primary 3D Cultures
Overall, this protocol guarantees flexibility and reliability, also providing useful guidelines for quality control checks to support different experimental settings. The setting up of a robust protocol for lung cancer PDO culture establishment and expansion is a key requirement for their employment both in cancer research and as predictive tools in clinical practice.
Authors
- Alessia Ciarrocchi (ORCID: https://orcid.org/0000-0002-5541-2075)
- Eleonora Zanetti (ORCID: https://orcid.org/0000-0002-3483-130X)
- Valentina Fantini (ORCID: https://orcid.org/0000-0003-1244-4453)
- Silvia Strocchi (ORCID: https://orcid.org/0000-0003-1248-4498)
- Giacomo Santandrea (ORCID: https://orcid.org/0000-0001-5487-0683)
- Vincenza Ylenia Cusenza (ORCID: https://orcid.org/0000-0002-4958-0419)
- Davide Nicoli (ORCID: https://orcid.org/0009-0000-6881-0366)
- A Grieco (ORCID: https://orcid.org/0009-0008-0531-4988)
- Massimiliano Paci (ORCID: https://orcid.org/0000-0001-7400-1792)
- Valentina Sancisi (ORCID: https://orcid.org/0000-0003-4357-1228)
- Giulio Verna
Institutions
- University of Modena and Reggio Emilia (IT)
- Azienda Sanitaria Unità Locale di Reggio Emilia (IT)
Publication Details
- Journal
- Cancers
- Published
- 2024-12-25
- DOI
- https://doi.org/10.3390/cancers17010027
- Citations
- 2
- Primary Topic
- Cancer Cells and Metastasis
- Type
- article
- Field-Weighted Citation Impact
- 0.57
Funders
- Ministero della Salute
- Fondazione Italiana per la Ricerca sul Cancro