PROTECTIVE ACTIVITY OF HYBRID DERIVATIVES OF TRITERPENOID COUMA-RINS AGAINST INFLUENZA AND HERPES VIRUSES

The aim of this study was to evaluate the protective properties of new hybrid deriva-tives of triterpenoid-coumarins using models of lethal influenza pneumonia and herpetic con-junctivitis. Materials and Methods. Animals. The study used two types of animals: female Balb/c mice (16-18 g) and male guinea pigs (400 g). The study employed the influenza virus strain A/California/07/09 (H1N1)pdm09, adapted to mice, from the collection of the Research Insti-tute of Influenza, A.A. Smorodintsev, Ministry of Health of Russia. Passage history: E4/E3M8E1. Conclusions. The protective activity of some hybrid derivatives of triterpenoid-coumarin was studied in the model of lethal influenza pneumonia caused by the influenza vi-rus and in the model of herpetic conjunctivitis during therapeutic and preventive use. It was shown that in the model of lethal influenza pneumonia using high doses of the influen-za virus, the use of all compounds improved the dynamics of animal weight, even if the animal mortality coincided with that of the control group. All the tested compounds, except for AV-49, positively affected the dynamics of death in infected animals. The most active compounds were AV-41, AV-42, and AV-53, with protection indices of 45%, 56%, and 34%, respective-ly. When studying the activity of compounds AV-41, AV-49, and AV-54 with lower doses of the virus, the protective properties of AV-41 and AV-53 were preserved at levels comparable to the reference drug oseltamivir. When investigating the protective activity of AV-42 and AV-53 in the model of her-petic conjunctivitis, it was shown that both drugs reduced the severity of disease symptoms (both keratitis and blepharitis), though less effectively than the reference drug acyclovir. Based on the overall protective activity in both viral models, the group of new hybrid triterpe-noid-coumarin derivatives appears promising for further antiviral research.