Tailored Functionalization of Plasmonic <scp>AgNPs</scp>/C:H:N:O Nanocomposite for Sensitive and Selective Detection
We report here on the development of tailored plasmonic AgNPs/C:H:N:O plasma polymer nanocomposites for the detection of the pathogenic bacterium Borrelia afzelii , with high selectivity and sensitivity. Silver (Ag) nanoparticles, generated by a gas aggregation source, are incorporated onto a C:H:N:O plasma polymer matrix, which is deposited by magnetron sputtering of a nylon 6.6. These anchored Ag nanoparticles propagate localized surface plasmon resonance (LSPR), optically responding to changes caused by immobilized pathogens near the nanoparticles. The tailored functionalization of AgNPs/C:H:N:O nanocomposite surface allows both high selectivity for the pathogen and high sensitivity with an LSPR red-shift Δλ > (4.20 ± 0.71) nm for 50 Borrelia per area 0.785 cm<sup>2</sup>. The results confirmed the ability of LSPR modulation for the rapid and early detection of (not only) tested pathogens.
Authors
- Ondřej Kylián (ORCID: https://orcid.org/0000-0001-6115-3471)
- Hana Mašková (ORCID: https://orcid.org/0000-0001-7798-5627)
- Ryan O. M. Rego (ORCID: https://orcid.org/0000-0001-6932-0940)
- Anna Kuzminova (ORCID: https://orcid.org/0000-0002-3723-608X)
- Vítězslav Straňák (ORCID: https://orcid.org/0000-0001-9676-634X)
- Ján Štěrba (ORCID: https://orcid.org/0000-0003-1005-1787)
- Lenka Doudova
- Sanjay Kumar (ORCID: https://orcid.org/0000-0003-1042-4491)
- Paval Curda
Institutions
- Charles University (CZ)
- University of South Bohemia in České Budějovice (CZ)
- Institute of Parasitology (CZ)
- Czech Academy of Sciences, Biology Centre (CZ)
Publication Details
- Journal
- Journal of Biophotonics
- Published
- 2024-12-23
- DOI
- https://doi.org/10.1002/jbio.202400353
- Citations
- 1
- Primary Topic
- Gold and Silver Nanoparticles Synthesis and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.12
Funders
- Grantová Agentura České Republiky