Biopriming of seeds with microbial biostimulant (Bacillus megaterium ) on improvement of seedling growth, biochemical and root traits of rice (Oryza sativa L.)

This study aimed to evaluate the potential of Bacillus megaterium in improvingseed germination and rice seedling's morpho-physiological andbiochemical traits. The experiment was conducted using a completely randomizeddesign to evaluate the effect of different concentrations of the biostimulantB. megaterium on rice seedlings growth and development undernursery conditions. Biopriming seeds with B. megaterium significantly enhancedseed germination and seedling traits, such as seedling shoot androot length, seedling height, number of leaves, seedling vigor, and shootand root dry biomass, compared to the untreated control. Among the treatments,biopriming seeds with B. megaterium at a concentration of 10g kg-1resulted in the greatest improvements across all the recorded parameters.Root traits such as total root length, surface area, average root diameter,root volume, and root tips and forks were also significantly enhanced. Additionally,biochemical changes like total chlorophyll and soluble protein contentshowed notable improvements with the concentration of B. megaterium. Hence, these results suggest that biopriming seeds with B. megateriumare an efficient strategy to improve seed germination and shoot and rootvigor in rice seedlings.