POSSIBLE WAYS OF MICROBIAL CONTAMINATION OF OIL AND FAT PRODUCTS PRESENTED IN KRASNOYARSK TRADE CHAINS

The purpose of the study is to identify possible ways of microbial contamination of oil and fat products in retail chains in Krasnoyarsk based on the analysis of the taxonomic composition of contaminant microorganisms. During the analysis of samples of margarine and vegetable oil, bacteria belonging to the families Micrococcaceae, Staphylococcaceae, Microbacteriaceae, Bacillaceae and Paenibacillaceae were identified. In one sample of margarine, coliform bacteria were found in an amount exceeding the standards by more than 125 times; in other samples of oil and fat products, coliform bacteria were not detected. Based on cultural-morphological properties and MALDI-TOF mass spectrometry in margarine samples, Bacillus atrophaeus Nakamura 1989, Curtobacterium flaccumfaciens (Hedges 1922) Collins and Jones 1984, Kocuria varians (Migula 1900) Stackebrandt et al. 1995, Paenibacillus sp., Staphyloccocus pasteuri Chesneau et al. 1993 and Staphylococcus epidermidis (Winslow & Winslow 1908) Evans 1916 were identified. Bacillus atrophaeus, Bacillus megaterium de Bary 1884, Bacillus pumilus Meyer and Gottheil 1901 (Approved Lists 1980), Micrococcus luteus (Schroeter 1872) Cohn 1872 emend were identified in vegetable oil samples. Wieser et al. 2002. The ecological features of the isolated bacterial species suggest that the microbial contamination of the analyzed samples of vegetable oil is due to the introduction of soil bacteria from the air. Microbial contamination of the analyzed samples of margarine is due to both the introduction of bacteria from the air and non-compliance with sanitary and hygienic requirements by personnel. This conclusion was made on the basis that margarine contaminating microorganisms are represented by both soil and human-associated bacterial species. Microorganisms-contaminants of vegetable oil are represented only by soil bacteria.