Counter-diffusion studies of human transthyretin: the growth of high-quality crystals for X-ray and neutron crystallography
A crystallogenesis study of human transthyretin using the counter-diffusion method is described as an alternative to conventional convective vapour diffusion and batch approaches for protein crystallization. The X-ray diffraction results show systematic trends that exhibit unique patterns of crystallization and high crystal quality as well as a remarkable degree of coherence within extended crystal rods that wholly fill the capillaries used. Preliminary neutron diffraction data have been recorded from a number of these samples, validating the feasibility of this methodology for neutron crystallography.
Authors
- Matthew P. Blakeley (ORCID: https://orcid.org/0000-0002-6412-4358)
- Michael Haertlein (ORCID: https://orcid.org/0000-0001-5410-0638)
- M.F. Oliva (ORCID: https://orcid.org/0000-0002-1204-1761)
- Edward P. Mitchell (ORCID: https://orcid.org/0000-0001-9809-9536)
- Matthew W. Bowler (ORCID: https://orcid.org/0000-0003-0465-3351)
- V. Trevor Forsyth (ORCID: https://orcid.org/0000-0003-0380-3477)
- Martine Moulin (ORCID: https://orcid.org/0000-0002-2212-4368)
- Clare De'Ath
Institutions
- Consejo Superior de Investigaciones Científicas (ES)
- Lund University (SE)
- European Synchrotron Radiation Facility (FR)
- Xerox (France) (FR)
- Instituto Andaluz de Ciencias de la Tierra (ES)
- European Molecular Biology Laboratory (FR)
- Institut Laue-Langevin (FR)
- Institut de Biologie Structurale (FR)
Publication Details
- Journal
- Journal of Applied Crystallography
- Published
- 2024-12-24
- DOI
- https://doi.org/10.1107/s1600576724011191
- Primary Topic
- Enzyme Structure and Function
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Université Grenoble Alpes
- Liam M. Kinne Foundation
- Human Frontier Science Program
- European Synchrotron Radiation Facility
- Vetenskapsrådet
- Ministerio de Ciencia e Innovación
- French Infrastructure for Integrated Structural Biology
- Erasmus+