Biological Synthesis of Silver Nanoparticles from <i>Juniperus procera</i> and <i>Azadirachta indica</i> Stem Extracts and Their Anticancer Properties

Silver nanoparticles (AgNPs) stand out in nanotechnology for their diverse properties as a pioneering field. The purpose of the study was to synthesize AgNPs using new approaches by using Juniperus procera ( J. procera ) and Azadirachta indica ( A. indica ) stem extracts and the synthesis was optimized by modifying factors including pH, concentration of the extract, temperature, and the intensity of light. Extract with 1 mL exposed to sunlight led to enhanced synthesis. By using UV-VIS spectroscopy, furrier transform infrared spectrometry, and field emission scan microscopy we identified functional groups. Also, we found that both J. procera and A. indica AgNPs had small sizes, 27 nm, and 67 nm, respectively. Further, these bioAgNPs as first attempts were highly cytotoxic towards A549, HCT116, and MCF7 cancer cells, suggesting they may prove to be potent cancer therapies in the future.