In pursuit of living cells: Dr. Bungo Wada and myself

Encountering the monograph ‘Analysis of Mitosis’ by Dr. Bungo Wada, published in 1966 as a supplementary issue of Cytologia, inspired me to contribute an article on my experiences with tobacco BY-2 cells and their synchronization. This is because Dr. Wada and I share a recognition of the importance of living cells. My studies have followed cytological changes during mitosis, using tobacco BY-2 cells and developing methods such as high synchronization and fluorescence tagging. I believe that my studies provide revelatory information about the cytoskeleton during the cell cycle. Through this brief comparison of our studies, I would like to remind people of the contributions of Dr. Wada and that Cytologia has space for mechanistic studies.