THE EFFECT OF BACTERIAL BIOINSECTICIDE ON THE MORPHOFUNCTIONAL STATUS OF HEMOCYTES BLAPTICA DUBIA

The purpose of the research is to analyze changes in the total hemocyte count and structure of their population, to assess the indicators of phagocytic activity in Blaptica dubia treated with an insecticide based on Bacillus thuringiensis, var. thuringiensis. Materials and methods. Quantitative studies of native hemocytes of adult Blaptica dubia using light microscopy were carried out. We determined phagocytic index and phagocytic number under conditions of bacterial load to evaluate the efficiency of foreign object absorption. The preparation Bitoxibacillin containing Bacillus thuringiensis, var. thuringiensis was selected as an insecticide. Results. We analyzed the effect of bacterial insecticide on the population structure of adult Blaptica dubia hemocytes. The hemolymph of ten insect specimens was studied. Seven hemocytic types were identified in adults: prohemocytes, plasmatocytes, granulocytes, vermicytes, spherulocytes, coagulocytes and crescent cells. On the third day after treatment with the insecticide, an increase in young forms of plasmatocytes and a sharp decrease in the number of prohemocytes and spherulocytes were noted. In this day, vermicites were absent in all specimens. The phagocytic index reached 53,2%, and the average number of bacteria absorbed by one phagocyte was 14,6 s.u. By the tenth day, a further decrease in the number of prohemocytes were recorded and plasmatocytes reached the size typical of mature cells. The values of phagocytic index and phagocytic number significantly decreased to 41% and 8 s.u., respectively.