Construction of Promoter Elements for Strong, Moderate, and Weak Gene Expression in Drosophila melanogaster
Based on our findings, we grouped the tested promoters into three categories: strong, moderate, and weak. These promoters can be utilized in transgenic model systems for protein production at different levels, from high to low. Bidirectional promoters, constructed "head-to-head", meaning oppositely directed with the minimum distance between them, represent a novel tool for the co-expression of proteins.
Authors
- Kseniya S. Kudryashova (ORCID: https://orcid.org/0000-0002-6937-3193)
- Oksana Maksimenko (ORCID: https://orcid.org/0000-0003-3502-0303)
- Igor S. Osadchiy (ORCID: https://orcid.org/0009-0005-7350-1054)
- Pavel Georgiev (ORCID: https://orcid.org/0000-0002-6509-3194)
- Irina O. Deriglazova
Institutions
- Institute of Gene Biology (RU)
Publication Details
- Journal
- Genes
- Published
- 2024-12-24
- DOI
- https://doi.org/10.3390/genes16010003
- Citations
- 2
- Primary Topic
- Animal Genetics and Reproduction
- Type
- article
- Field-Weighted Citation Impact
- 1.10
Funders
- Ministry of Education and Science of the Russian Federation