Genotyping of Yersinia pseudotuberculosis Strains Isolated in Russia Using MLST

The aim of this study was the genotyping of Yersinia pseudotuberculosis strains isolated in Russia using multilocus sequence typing (MLST), a comparative analysis of MLST results with INDEL typing results. Materials and methods . 348 strains of various serotypes isolated in different regions of the world, including 59 Russian strains, were analyzed. 302 strains from the NCBI database were used. The nucleotide sequences of the strains were determined using the MiSeq technology platform. The author MLSTtyper program was used for MLST analysis. Results and discussion . It has been found out that strains of at least, 2, 9, 19, 26, 32, 42, 43 ST-types circulate in Russia. The majority of the studied Russian strains belong to the ST2 type (58 %). Phylogenetic analysis conducted using MLST data showed that a number of clusters contains strains that have been circulating in Russia for a long time, and strains of these genotypes are not found abroad. Some of the Russian strains are included in clusters containing strains isolated in various regions of the world and can be attributed to strains of either “Asian” or “European” types. The data obtained using the MLSTtyper program coincided with the data of foreign authors when analyzing the same strains using the MLST method. Comparison of the results of genetic differentiation of Y. pseudotuberculosis strains using MLST and INDEL typing indicates that MLST has a greater discriminatory ability; however, in some cases INDEL genotyping allows differentiating those strains that had the same ST genotype.